Structure and Function of Organisms
Lab 2: Microscopy I
Questions for First Microscopy Lab
1. The ability to distinguish two points in the specimen (i.e. see two points, instead of one) relates to a problem in using light microscopes termed _______________.
2. The numerical aperature of an objective lens determines the ability of that lens to ________ portions of the specimen.
3. A stain is used to induce ______________ in the specimen.
4. The smallest element in a digital picture is refered to as a _____________.
5. The total magnification through a compound microscope is equal to the magnification of the ___________ times the magnification of the _________________.
6. Oil immersion improves light microscopy by increasing the _________________ of the medium between the objective lens and the specimen.
7. ____________________ refers to the ability to distinguish objects on each side of the plane of focus.
8. A microscopic technique used to increase contrast in an unstained specimen is ________________.
9. _______________ is the stage of mitosis in which the chromatin material begins to condense into individual chromosomes and the nuclear membrane begins to disappear.
10. _________ is the stage of mitosis when the sister chromatids begin to separate to opposite poles.
11. _________ is the stage of mitosis in which cytokinesis, the separation of the cytoplasm to the two daughter cells, occurs.
12. The objective lenses are held in a structure on the microscope known as the ___________.
13. The protozoan you will study in lab this week is _______________________.
14. The source of the tissue you will use to study mitosis is ____________________.
15. The specimen to be examined with a microscope is placed on what part of the microscope? ________.
16. In the lab you will make scrappings of your __________ to look at epithelial cells.
17. The stain aceto-orcein is used to stain what material inside of cells? __________
18. The lens system located between the specimen and the ocular lens is known as the _____________.
19. What power of objective lens is usually used with oil immersion? __________
20. Scopes with a single eyepiece said to be monocular. What are scopes with two eyepieces said to be? ________________
21. Working distance is said to be that distance between the objective lens and the specimen when the specimen is ____________ for your eyes.
22. Which lens is likely to have the largest (longest) working distance? The 10X objective or the 100X objective? _______________
23. The HCl (hydrochloric acid) is used to break down the ____________ of the cells used to study mitosis.
24. To view the specimen on the microscope, the observer looks through the ___________ lens.
25. When you focus your microscope, you change the distance between the objective and the _________.
26. The symbol lambda in Abbe?s equation refers to the ______________ of light.
27. A difference in the absorption of light by different portions of the specimen is the basis for ___________ (choose ?brightfield? or ?phase?) microscopy.
28. The illuminator for the microscope is located in the part of the microscope known as the ________.
29. The angle theta in Abbe?s equation is _______ of the angle of the cone of light entering the microscope objective.
30. Mitosis is found in what portion of the root? ___________